Journal: Frontiers in Pharmacology

Article Title: α4-Containing GABA A Receptors are Required for Antagonism of Ethanol-Induced Motor Incoordination and Hypnosis by the Imidazobenzodiazepine Ro15-4513

doi: 10.3389/fphar.2011.00018

Figure Lengend Snippet: Ro15-4513 antagonism of ethanol-induced motor ataxia . (A) Performance of WT and GABA A -Rα4 subunit KO mice on fixed speed (8 rpm) rotarod following pretreatment with 2 g/kg ethanol 5 min prior to treatment with 10 mg/kg Ro15-4513 or vehicle.* p ≤ 0.01 – WT ethanol/vehicle vs WT ethanol/Ro15-4513. (B) AUCs of response curves in (A) . Effect of genotype, treatment, and the interaction were not significant. (C) Performance on fixed speed (12 rpm) rotarod following treatment with 1.5 g/kg ethanol ±3 mg/kg Ro15-4513. * p < 0.005, ** p < 0.0001 – WT ethanol vs WT ethanol + Ro15-4513. (D) AUCs of response curves in (C) . WT and KO mice did not differ following injection of ethanol alone. Ro15-4513 antagonized ethanol-induced motor ataxia in WT, but not in KO mice. All values are expressed as mean ± SEM.

Article Snippet: Ro15-4513 was freshly prepared in 0.3% Tween-80 in saline at 1 mg/ml on the morning of the experiment in BD Falcon round bottomed tubes.

Techniques: Injection

Journal: Frontiers in Pharmacology

Article Title: α4-Containing GABA A Receptors are Required for Antagonism of Ethanol-Induced Motor Incoordination and Hypnosis by the Imidazobenzodiazepine Ro15-4513

doi: 10.3389/fphar.2011.00018

Figure Lengend Snippet: Antagonism of ethanol (3. 5 g/kg)-induced LORR by Ro15-4513 (20 mg/kg) . (A) In male mice, Ro15-4513 antagonized the duration of ethanol-induced LORR in WT, but not in KO. (B) In female mice, Ro15-4513 antagonized ethanol-induced LORR ( p < 0.0001) irrespective of genotype. All values are expressed as mean ± SEM.

Article Snippet: Ro15-4513 was freshly prepared in 0.3% Tween-80 in saline at 1 mg/ml on the morning of the experiment in BD Falcon round bottomed tubes.

Techniques: